› 5gli

X-ray diffraction
2.5Å resolution

Human endothelin receptor type-B in the ligand-free form

Released:
DOI: 10.2210/pdb5gli/pdb
PDB entry 5gli coloured by chain, front view.
PDB entry 5gli coloured by chain, side view.
PDB entry 5gli coloured by chain, top view.
Source organisms:
Primary publication:
Activation mechanism of endothelin ETB receptor by endothelin-1.
Shihoya W, Nishizawa T, Okuta A, Tani K, Dohmae N, Fujiyoshi Y, Nureki O, Doi T
Nature 537 363-368 (2016)
PMID: 27595334

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Endothelin receptor type B Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 52.08 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24530 (Residues: 65-304, 305-306, 311-407; Coverage: 82%)
  • Canonical: P00720 (Residues: 61-161; Coverage: 62%)
Gene names: E, EDNRB, ETRB
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand OLC 2 x OLC
Ligand OLA 1 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 73.98Å b: 147.54Å c: 107.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.276
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

How Ligands Illuminate GPCR Molecular Pharmacology.
Wacker et al. (2017)
19 more

17 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
16 more