› 5gif

X-ray diffraction
1.3Å resolution

Crystal Structure of Drosophila melanogaster E47D Dopamine N-Acetyltransferase in Binary Complex with Acetyl-CoA

Released:
DOI: 10.2210/pdb5gif/pdb
PDB entry 5gif coloured by chain, front view.
PDB entry 5gif coloured by chain, side view.
PDB entry 5gif coloured by chain, top view.
Source organism: Drosophila melanogaster
Entry authors: Yang YC, Wu CY, Cheng HC, Lyu PC

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arylalkylamine N-acetyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.43 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q94521 (Residues: 56-265; Coverage: 76%)
Gene names: AANAT1, CG3318, Dat, NAT1, speck
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand D1D 2 x D1D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 44.138Å b: 56.58Å c: 84.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.186
Expression system: Escherichia coli K-12

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