5g05

Electron Microscopy
3.5Å resolution

Cryo-EM structure of combined apo phosphorylated APC

Released:
Related structures: EMD-3388

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only icosamer (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
ANAPHASE-PROMOTING COMPLEX SUBUNIT 1 Chain: A
Molecule details ›
Chain: A
Length: 1944 amino acids
Theoretical weight: 216.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9H1A4 (Residues: 1-1944; Coverage: null%)
Gene names: ANAPC1, TSG24
ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 Chain: B
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NYG5 (Residues: 1-84; Coverage: null%)
Gene names: ANAPC11, HSPC214
CELL DIVISION CYCLE PROTEIN 23 HOMOLOG Chains: C, P
Molecule details ›
Chains: C, P
Length: 597 amino acids
Theoretical weight: 68.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UJX2 (Residues: 1-597; Coverage: null%)
Gene names: ANAPC8, CDC23
ANAPHASE-PROMOTING COMPLEX SUBUNIT 15 Chain: D
Molecule details ›
Chain: D
Length: 121 amino acids
Theoretical weight: 14.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P60006 (Residues: 1-121; Coverage: null%)
Gene names: ANAPC15, C11orf51, HSPC020
ANAPHASE-PROMOTING COMPLEX SUBUNIT 16 Chain: E
Molecule details ›
Chain: E
Length: 110 amino acids
Theoretical weight: 11.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96DE5 (Residues: 1-110; Coverage: null%)
Gene names: ANAPC16, C10orf104, CENP-27
CELL DIVISION CYCLE PROTEIN 27 HOMOLOG Chains: F, H
Molecule details ›
Chains: F, H
Length: 824 amino acids
Theoretical weight: 91.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30260 (Residues: 1-824; Coverage: null%)
Gene names: ANAPC3, CDC27, D0S1430E, D17S978E
ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26 Chains: G, W
Molecule details ›
Chains: G, W
Length: 85 amino acids
Theoretical weight: 9.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8NHZ8 (Residues: 1-85; Coverage: null%)
Gene names: ANAPC12, C9orf17, CDC26
ANAPHASE-PROMOTING COMPLEX SUBUNIT 4 Chain: I
Molecule details ›
Chain: I
Length: 808 amino acids
Theoretical weight: 92.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UJX5 (Residues: 1-808; Coverage: null%)
Gene names: ANAPC4, APC4
CELL DIVISION CYCLE PROTEIN 16 HOMOLOG Chains: J, K
Molecule details ›
Chains: J, K
Length: 620 amino acids
Theoretical weight: 71.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13042 (Residues: 1-620; Coverage: null%)
Gene names: ANAPC6, CDC16
ANAPHASE-PROMOTING COMPLEX SUBUNIT 10 Chain: L
Molecule details ›
Chain: L
Length: 185 amino acids
Theoretical weight: 21.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UM13 (Residues: 1-185; Coverage: null%)
Gene names: ANAPC10, APC10
ANAPHASE-PROMOTING COMPLEX SUBUNIT 13 Chain: M
Molecule details ›
Chain: M
Length: 74 amino acids
Theoretical weight: 8.53 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q2NKV2 (Residues: 1-74; Coverage: null%)
Gene name: ANAPC13
ANAPHASE-PROMOTING COMPLEX SUBUNIT 2 Chain: N
Molecule details ›
Chain: N
Length: 822 amino acids
Theoretical weight: 93.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UJX6 (Residues: 1-822; Coverage: null%)
Gene names: ANAPC2, APC2, KIAA1406
ANAPHASE-PROMOTING COMPLEX SUBUNIT 5 Chain: O
Molecule details ›
Chain: O
Length: 755 amino acids
Theoretical weight: 85.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UJX4 (Residues: 1-755; Coverage: null%)
Gene names: ANAPC5, APC5
UNIDENTIFIED PEPTIDE Chain: T
Molecule details ›
Chain: T
Length: 15 amino acids
Theoretical weight: 1.18 KDa
Source organism: unidentified
Expression system: Spodoptera frugiperda
ANAPHASE-PROMOTING COMPLEX SUBUNIT 7 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 599 amino acids
Theoretical weight: 66.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UJX3 (Residues: 1-599; Coverage: null%)
Gene names: ANAPC7, APC7

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-3388
Expression system: Spodoptera frugiperda