› 5g00

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A POTATO STI-KUNITZ BIFUNCTIONAL INHIBITOR OF SERINE AND ASPARTIC PROTEASES IN SPACE GROUP P4322 AND PH 7.4

Released:
DOI: 10.2210/pdb5g00/pdb
PDB entry 5g00 coloured by chain, front view.
PDB entry 5g00 coloured by chain, side view.
PDB entry 5g00 coloured by chain, top view.
Source organism: Solanum tuberosum
Primary publication:
Structures of a bi-functional Kunitz-type STI family inhibitor of serine and aspartic proteases: Could the aspartic protease inhibition have evolved from a canonical serine protease-binding loop?
Guerra Y, Valiente PA, Pons T, Berry C, Rudiño-Piñera E
J Struct Biol 195 259-271 (2016)
PMID: 27329566

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartic protease inhibitor 5 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 20.53 KDa
Source organism: Solanum tuberosum
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: M1AKE5 (Residues: 32-218; Coverage: 94%)
Gene names: 102604582, API5
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P4322
Unit cell:
a: 77.18Å b: 77.18Å c: 95.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.244
Expression system: Komagataella phaffii GS115

Quick links

Citations

1 review citation

Protease Inhibitors from Plants as Therapeutic Agents- A Review.
Cid-Gallegos et al. (2022)
2 other articles