5fu7

X-ray diffraction
3.1Å resolution

drosophila nanos NBR peptide bound to the NOT module of the human CCR4-NOT complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CCR4-NOT transcription complex subunit 1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 535 amino acids
Theoretical weight: 61.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 1833-2361; Coverage: 22%)
  • Best match: A5YKK6-3 (Residues: 1828-2146)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-Not complex component, Not1
CCR4-NOT transcription complex subunit 2 Chains: B, F
Molecule details ›
Chains: B, F
Length: 197 amino acids
Theoretical weight: 22.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZN8 (Residues: 350-540; Coverage: 35%)
Gene names: CDC36, CNOT2, HSPC131, MSTP046, NOT2
Sequence domains: NOT2 / NOT3 / NOT5 family
Structure domains: SH3 type barrels.
CCR4-NOT transcription complex subunit 3 Chains: C, G
Molecule details ›
Chains: C, G
Length: 148 amino acids
Theoretical weight: 18.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75175 (Residues: 607-748; Coverage: 19%)
Gene names: CNOT3, KIAA0691, LENG2, NOT3
Sequence domains: NOT2 / NOT3 / NOT5 family
Structure domains: SH3 type barrels.
Nanos-type domain-containing protein Chains: D, H
Molecule details ›
Chains: D, H
Length: 54 amino acids
Theoretical weight: 5.75 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B4KGY5 (Residues: 116-163; Coverage: 13%)
Gene names: CG5637, DRONANOS, Dmel\CG5637, Dmel_CG5637, Dronanos, NANOS, NOS, Nanos, Nos, l(3)07117, l(3)j3B6, l(3)j4B6, nNOS, nos

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 75.95Å b: 135.67Å c: 104.97Å
α: 90° β: 107.98° γ: 90°
R-values:
R R work R free
0.168 0.165 0.227
Expression system: Escherichia coli BL21(DE3)