5ft4

X-ray diffraction
2Å resolution

Crystal structure of the cysteine desulfurase CsdA from Escherichia coli at 1.996 Angstroem resolution

Released:

Function and Biology Details

Reactions catalysed:
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase CsdA Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46925 (Residues: 1-401; Coverage: 100%)
Gene names: JW2781, b2810, csdA, ygdJ
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 64.095Å b: 102.561Å c: 71.258Å
α: 90° β: 114.45° γ: 90°
R-values:
R R work R free
0.14 0.138 0.191
Expression system: Escherichia coli BL21(DE3)