› 5fsj

X-ray diffraction
1.2Å resolution

Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure

Released:
DOI: 10.2210/pdb5fsj/pdb
PDB entry 5fsj coloured by chain, front view.
PDB entry 5fsj coloured by chain, side view.
PDB entry 5fsj coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Primary publication:
Gas-Sensitive Biological Crystals Processed in Pressurized Oxygen and Krypton Atmospheres: Deciphering Gas Channels in Proteins Using a Novel `Soak-and-Freeze' Methodology.
Lafumat B, Mueller-Dieckmann C, Colloc'h N, Prange T, Royant A, van der Linden P, Carpentier P
J Appl Crystallogr 49 1478- (2016)

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand VAL 1 x VAL
Ligand LYS 1 x LYS
Ligand CA 5 x CA
Ligand ZN 2 x ZN
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 92.922Å b: 92.922Å c: 130.252Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.105 0.104 0.122
Expression system: Escherichia coli

Quick links