5frx

X-ray diffraction
2.4Å resolution

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-nitrophenol

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sigma-54 factor interaction domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.5 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O84957 (Residues: 1-211; Coverage: 37%)
Gene name: poxR
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 46.241Å b: 70.47Å c: 105.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.206 0.288
Expression system: Escherichia coli BL21(DE3)