› 5fr8

X-ray diffraction
2.83Å resolution

Crystal structure of the siderophore receptor PirA from Acinetobacter baumannii

Released:
DOI: 10.2210/pdb5fr8/pdb
PDB entry 5fr8 coloured by chain, front view.
PDB entry 5fr8 coloured by chain, side view.
PDB entry 5fr8 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii.
Moynié L, Luscher A, Rolo D, Pletzer D, Tortajada A, Weingart H, Braun Y, Page MG, Naismith JH, Köhler T
Antimicrob Agents Chemother 61 (2017)
PMID: 28137795

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable TonB-dependent siderophore receptor PirA Chains: A, B
Molecule details ›
Chains: A, B
Length: 733 amino acids
Theoretical weight: 80.59 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0C8V9 (Residues: 25-754; Coverage: 100%)
Gene names: A1S_0980, HMPREF0010_01517, pirA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 99.09Å b: 99.09Å c: 504.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.227 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Cefiderocol: Discovery, Chemistry, and In Vivo Profiles of a Novel Siderophore Cephalosporin.
Sato et al. (2019)
6 more

4 mentions without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
3 more