› 5foy

X-ray diffraction
2.25Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139087 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Binary larvicide subunit BinA Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 41.96 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Bacillus thuringiensis serovar israelensis
UniProt:
  • Canonical: P06575 (Residues: 1-370; Coverage: 100%)
Gene name: binA
Sequence domains: Insecticidal Crystal Toxin, P42
Binary larvicide subunit BinB Chain: B
Molecule details ›
Chain: B
Length: 448 amino acids
Theoretical weight: 51.49 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Bacillus thuringiensis serovar israelensis
UniProt:
  • Canonical: P10565 (Residues: 1-448; Coverage: 100%)
Gene names: binB, sph04
Sequence domains: Insecticidal Crystal Toxin, P42
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P212121
Unit cell:
a: 86.887Å b: 97.416Å c: 128.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.2
Expression system: Bacillus thuringiensis serovar israelensis

Quick links

Citations

16 review citations

X-Ray Free-Electron Lasers for the Structure and Dynamics of Macromolecules.
Chapman HN. (2019)
15 more

5 mentions without citation

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure.
Colletier et al. (2016)
4 more