X-ray diffraction
2.3Å resolution

Crystal structure of hte Cryptosporidium muris cytosolic leucyl-tRNA synthetase editing domain complex with a post-transfer editing analogue of norvaline (Nv2AA)


Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Leucyl-tRNA synthetase family protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.97 KDa
Source organism: Cryptosporidium muris RN66
Expression system: Escherichia coli K-12
  • Canonical: B6AA20 (Residues: 254-541; Coverage: 26%)
Gene name: CMU_041310

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 107.73Å b: 107.73Å c: 309.51Å
α: 90° β: 90° γ: 90°
R R work R free
0.203 0.201 0.236
Expression system: Escherichia coli K-12