PDBe 5fn3

Electron Microscopy
4.1Å resolution

Cryo-EM structure of gamma secretase in class 1 of the apo- state ensemble

Released:
Source organism: Homo sapiens
Related structures: EMD-3238

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 78.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-709; Coverage: 100%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Sequence domains:
Presenilin-1 Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names: AD3, PS1, PSEN1, PSNL1
Sequence domains: Presenilin
Gamma-secretase subunit APH-1A Chain: C
Molecule details ›
Chain: C
Length: 265 amino acids
Theoretical weight: 29.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96BI3 (Residues: 1-265; Coverage: 100%)
Gene names: APH1A, CGI-78, PSF, UNQ579/PRO1141
Sequence domains: Aph-1 protein
Gamma-secretase subunit PEN-2 Chain: D
Molecule details ›
Chain: D
Length: 101 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NZ42 (Residues: 1-101; Coverage: 100%)
Gene names: MDS033, PEN2, PSENEN
Sequence domains: Presenilin enhancer-2 subunit of gamma secretase
POLY ALA CHAIN Chain: G
Molecule details ›
Chain: G
Length: 24 amino acids
Theoretical weight: 1.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-3238
Expression system: Homo sapiens