› › Structure analysis

Structure of a nuclease-deletion mutant of the Type ISP restriction-modification enzyme LlaGI in complex with a DNA substrate mimic

X-ray diffraction
2.84Å resolution
PDB entry 5ffj coloured by chain, front view.
PDB entry 5ffj coloured by chain, side view.
PDB entry 5ffj coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: site-specific DNA-methyltransferase (adenine-specific) and DNA
hide full assembly name
Multimeric state: hetero trimer
Accessible surface area: 50064.43 Å2
Buried surface area: 7205.58 Å2
Dissociation area: 2,087.74 Å2
Dissociation energy (ΔGdiss): 9.7 kcal/mol
Dissociation entropy (TΔSdiss): 12.49 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118672
Assembly 2
Download    3D Visualisation
Assembly Name: site-specific DNA-methyltransferase (adenine-specific) and DNA
hide full assembly name
Multimeric state: hetero trimer
Accessible surface area: 58123.1 Å2
Buried surface area: 7270.76 Å2
Dissociation area: 2,665.1 Å2
Dissociation energy (ΔGdiss): 9.19 kcal/mol
Dissociation entropy (TΔSdiss): 13.72 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118672

Macromolecules

site-specific DNA-methyltransferase (adenine-specific)
Chains: A, B
Length: 1406 amino acids
Theoretical weight: 160.72 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93R01 (Residues: 166-1570; Coverage: 90%)
Pfam:
InterPro:
site-specific DNA-methyltransferase (adenine-specific) in PDB entry 5ffj, assembly 1, front view.
site-specific DNA-methyltransferase (adenine-specific) in PDB entry 5ffj, assembly 1, side view.
site-specific DNA-methyltransferase (adenine-specific) in PDB entry 5ffj, assembly 1, top view.
Molecule details ›

Search similar proteins

DNA chains D, E
Name: DNA (5'-D(P*TP*CP*CP*TP*CP*CP*AP*TP*CP*CP*AP*GP*TP*CP*TP*AP*TP*TP*AP*GP*CP*T)-3')
Representative chains: D, E
Source organism: unidentified [32644]
Expression system: Not provided
Length: 23 nucleotides
Theoretical weight: 6.93 KDa
DNA (5'-D(P*TP*CP*CP*TP*CP*CP*AP*TP*CP*CP*AP*GP*TP*CP*TP*AP*TP*TP*AP*GP*CP*T)-3') in PDB entry 5ffj, assembly 1, front view.
DNA (5'-D(P*TP*CP*CP*TP*CP*CP*AP*TP*CP*CP*AP*GP*TP*CP*TP*AP*TP*TP*AP*GP*CP*T)-3') in PDB entry 5ffj, assembly 1, side view.
DNA (5'-D(P*TP*CP*CP*TP*CP*CP*AP*TP*CP*CP*AP*GP*TP*CP*TP*AP*TP*TP*AP*GP*CP*T)-3') in PDB entry 5ffj, assembly 1, top view.
Molecule details ›

Search similar DNA

DNA chains C, F
Name: DNA (5'-D(P*TP*AP*GP*CP*TP*AP*AP*TP*AP*GP*AP*CP*TP*GP*GP*AP*TP*GP*GP*AP*GP*G)-3')
Representative chains: C, F
Source organism: unidentified [32644]
Expression system: Not provided
Length: 23 nucleotides
Theoretical weight: 7.18 KDa
DNA (5'-D(P*TP*AP*GP*CP*TP*AP*AP*TP*AP*GP*AP*CP*TP*GP*GP*AP*TP*GP*GP*AP*GP*G)-3') in PDB entry 5ffj, assembly 1, front view.
DNA (5'-D(P*TP*AP*GP*CP*TP*AP*AP*TP*AP*GP*AP*CP*TP*GP*GP*AP*TP*GP*GP*AP*GP*G)-3') in PDB entry 5ffj, assembly 1, side view.
DNA (5'-D(P*TP*AP*GP*CP*TP*AP*AP*TP*AP*GP*AP*CP*TP*GP*GP*AP*TP*GP*GP*AP*GP*G)-3') in PDB entry 5ffj, assembly 1, top view.
Molecule details ›

Search similar DNA

Quick links