5fey

X-ray diffraction
2.23Å resolution

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase TRIM32 Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13049 (Residues: 7-93; Coverage: 13%)
Gene names: HT2A, TRIM32
Sequence domains: RING-type zinc-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 54.536Å b: 54.536Å c: 99.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.248 0.273
Expression system: Escherichia coli