› 5fbh

X-ray diffraction
2.7Å resolution

Crystal structure of the extracellular domain of human calcium sensing receptor with bound Gd3+

Released:
DOI: 10.2210/pdb5fbh/pdb
PDB entry 5fbh coloured by chain, front view.
PDB entry 5fbh coloured by chain, side view.
PDB entry 5fbh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for regulation of human calcium-sensing receptor by magnesium ions and an unexpected tryptophan derivative co-agonist.
Zhang C, Zhang T, Zou J, Miller CL, Gorkhali R, Yang JY, Schilmiller A, Wang S, Huang K, Brown EM, Moremen KW, Hu J, Yang JJ
Sci Adv 2 e1600241 (2016)
PMID: 27386547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular calcium-sensing receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 568 amino acids
Theoretical weight: 64.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41180 (Residues: 20-541; Coverage: 49%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

6 bound ligands:
Ligand GD3 2 x GD3
Ligand MG 3 x MG
Ligand BCT 2 x BCT
Ligand CL 2 x CL
Ligand NAG 7 x NAG
Ligand TCR 2 x TCR
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 172.107Å b: 83.106Å c: 94.47Å
α: 90° β: 105.15° γ: 90°
R-values:
R R work R free
0.184 0.182 0.236
Expression system: Homo sapiens

Quick links

Citations

24 review citations

Disorders of the calcium-sensing receptor and partner proteins: insights into the molecular basis of calcium homeostasis.
Hannan et al. (2016)
23 more

10 mentions without citation

International Union of Basic and Clinical Pharmacology. CVIII. Calcium-Sensing Receptor Nomenclature, Pharmacology, and Function.
Leach et al. (2020)
9 more