X-ray diffraction
2.81Å resolution

Crystal structure of the alpha subunit of glycyl tRNA synthetase (GlyRS) from Aquifex aeolicus in complex with an analog of glycyl adenylate (Gly-SA)


Function and Biology Details

Reaction catalysed:
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Glycine--tRNA ligase alpha subunit Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 299 amino acids
Theoretical weight: 35.55 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O67081 (Residues: 1-285; Coverage: 100%)
Gene names: aq_945, glyQ
Sequence domains: Glycyl-tRNA synthetase alpha subunit
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Unit cell:
a: 101.829Å b: 130.01Å c: 145.52Å
α: 90° β: 90° γ: 90°
R R work R free
0.24 0.239 0.252
Expression system: Escherichia coli BL21(DE3)