› 5f3b

X-ray diffraction
1.76Å resolution

Structure of myostatin in complex with chimeric RK35 antibody

Released:
DOI: 10.2210/pdb5f3b/pdb
PDB entry 5f3b coloured by chain, front view.
PDB entry 5f3b coloured by chain, side view.
PDB entry 5f3b coloured by chain, top view.
Source organisms:
Primary publication:
Beyond CDR-grafting: Structure-guided humanization of framework and CDR regions of an anti-myostatin antibody.
Apgar JR, Mader M, Agostinelli R, Benard S, Bialek P, Johnson M, Gao Y, Krebs M, Owens J, Parris K, St Andre M, Svenson K, Morris C, Tchistiakova L
MAbs 8 1302-1318 (2016)
PMID: 27625211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229744 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RK35 Chimeric antibody heavy chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 221 amino acids
Theoretical weight: 23.55 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
RK35 Chimeric antibody light chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 213 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Growth/differentiation factor 8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 109 amino acids
Theoretical weight: 12.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: O14793 (Residues: 267-375; Coverage: 31%)
Gene names: GDF8, MSTN
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 66.09Å b: 67.616Å c: 78.559Å
α: 78.98° β: 88.77° γ: 67.44°
R-values:
R R work R free
0.181 0.179 0.206
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy.
Almagro et al. (2017)
4 more

7 mentions without citation

Structural basis for potency differences between GDF8 and GDF11.
Walker et al. (2017)
6 more