› 5f2t

X-ray diffraction
2.06Å resolution

Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - C 2 space group

Released:
DOI: 10.2210/pdb5f2t/pdb
PDB entry 5f2t coloured by chain, front view.
PDB entry 5f2t coloured by chain, side view.
PDB entry 5f2t coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.
Albesa-Jové D, Svetlíková Z, Tersa M, Sancho-Vaello E, Carreras-González A, Bonnet P, Arrasate P, Eguskiza A, Angala SK, Cifuente JO, Korduláková J, Jackson M, Mikušová K, Guerin ME
Nat Commun 7 10906 (2016)
PMID: 26965057

Function and Biology Details

Reaction catalysed: 
An acyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol = CoA + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol mannoside acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 34.3 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QWG5 (Residues: 13-304; Coverage: 96%)
Gene names: MSMEG_2934, MSMEI_2860, patA
Sequence domains: Bacterial lipid A biosynthesis acyltransferase

Ligands and Environments

2 bound ligands:
Ligand PLM 2 x PLM
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 93.6Å b: 71.09Å c: 76.25Å
α: 90° β: 91.95° γ: 90°
R-values:
R R work R free
0.176 0.173 0.23
Expression system: Mycolicibacterium smegmatis MC2 155

Quick links

Citations

2 review citations

How lipid droplets "TAG" along: Glycerolipid synthetic enzymes and lipid storage.
Wang et al. (2017)
1 more

2 mentions without citation

Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.
Albesa-Jové et al. (2016)
1 more