5f28

X-ray diffraction
2.9Å resolution

Crystal structure of FAT domain of Focal Adhesion Kinase (FAK) bound to the transcription factor MEF2C

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero tetradecamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myocyte-specific enhancer factor 2C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 95 amino acids
Theoretical weight: 11.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CFN5 (Residues: 1-95; Coverage: 20%)
  • Best match: Q8CFN5-4 (Residues: 1-95)
Gene name: Mef2c
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
Focal adhesion kinase 1 Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 149 amino acids
Theoretical weight: 16.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34152 (Residues: 904-1052; Coverage: 14%)
Gene names: Fadk, Fak, Fak1, Kiaa4203, Ptk2
Sequence domains: Focal adhesion targeting region
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P42212
Unit cell:
a: 139.21Å b: 139.21Å c: 90.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.235
Expression system: Escherichia coli BL21(DE3)