› 5eym

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 40.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02750 (Residues: 35-393; Coverage: 91%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 5U5 2 x 5U5
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P61
Unit cell:
a: 78.76Å b: 78.76Å c: 223.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.243 0.308
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Second-Generation Antimitotics in Cancer Clinical Trials.
Novais et al. (2021)
2 more

6 mentions without citation

Quercetin conjugated with superparamagnetic iron oxide nanoparticles improves learning and memory better than free quercetin via interacting with proteins involved in LTP.
Amanzadeh et al. (2019)
5 more