5eyb

X-ray diffraction
2.7Å resolution

X-ray Structure of Reb1-Ter Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein reb1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 43.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P6H9 (Residues: 146-504; Coverage: 71%)
Gene names: SPBC1198.11c, SPBC660.01c, reb1
Sequence domains: Myb-like DNA-binding domain
DNA (26-MER) Chains: C, E
Molecule details ›
Chains: C, E
Length: 26 nucleotides
Theoretical weight: 8.09 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (26-MER) Chains: D, F
Molecule details ›
Chains: D, F
Length: 26 nucleotides
Theoretical weight: 7.88 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 68.959Å b: 162.911Å c: 71.086Å
α: 90° β: 94.66° γ: 90°
R-values:
R R work R free
0.212 0.21 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided