PDBe 5ew5

X-ray diffraction
3.2Å resolution

Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 582 amino acids
Theoretical weight: 61.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09883 (Residues: 1-582; Coverage: 100%)
Gene names: cei, col
Sequence domains:
Colicin-E9 immunity protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 94 amino acids
Theoretical weight: 10.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 94.894Å b: 116.05Å c: 150.365Å
α: 90° β: 93.72° γ: 90°
R-values:
R R work R free
0.215 0.212 0.271
Expression system: Escherichia coli BL21(DE3)