5eul

X-ray diffraction
3.7Å resolution

Structure of the SecA-SecY complex with a translocating polypeptide substrate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-207587 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 836 amino acids
Theoretical weight: 94.36 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P28366 (Residues: 1-741, 742-745, 746-780; Coverage: 93%)
Gene names: BSU35300, div+, secA
Sequence domains:
Protein translocase subunit SecY Chain: Y
Molecule details ›
Chain: Y
Length: 424 amino acids
Theoretical weight: 46.77 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli
UniProt:
  • Canonical: A4IJK8 (Residues: 1-430; Coverage: 99%)
Gene names: GTNG_0125, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 70 amino acids
Theoretical weight: 8.25 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli
UniProt:
  • Canonical: A4IJH4 (Residues: 1-60; Coverage: 100%)
Gene names: GTNG_0091, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
AYC08 Chain: V
Molecule details ›
Chain: V
Length: 131 amino acids
Theoretical weight: 14.48 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 127.798Å b: 127.798Å c: 554.772Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.296 0.295 0.315
Expression system: Escherichia coli