› 5ete

X-ray diffraction
2.1Å resolution

Structure of pathogen-related yeast protein, Pry1 in complex with a competitive inhibitor of cholesterol binding

Released:
DOI: 10.2210/pdb5ete/pdb
PDB 5ete coloured by chain and viewed from the front.
PDB 5ete coloured by chain and viewed from the side.
PDB 5ete coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae YJM1434
Primary publication:
Structural and functional characterization of the CAP domain of pathogen-related yeast 1 (Pry1) protein.
Darwiche R, Kelleher A, Hudspeth EM, Schneiter R, Asojo OA
OpenAccess logo Sci Rep 6 28838 (2016)
PMID: 27344972

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pry1p Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 15.9 KDa
Source organism: Saccharomyces cerevisiae YJM1434
Expression system: Pichia
Structure domains: CAP

Ligands and Environments

1 bound ligand:
Ligand DIO 4 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P6222
Unit cell:
a: 124.74Å b: 124.74Å c: 59.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.19
Expression system: Pichia

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Citations

2 review citations

The function of yeast CAP family proteins in lipid export, mating, and pathogen defense.
Darwiche et al. (2018)
1 more