5erm

X-ray diffraction
2.3Å resolution

Crystal structure of cyclization domain of Phomopsis amygdali fusicoccadiene synthase complexed with magnesium ions and pamidronate

Released:

Function and Biology Details

Reactions catalysed:
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Geranylgeranyl diphosphate = fusicocca-2,10(14)-diene + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-106916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fusicoccadiene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 41.69 KDa
Source organism: Diaporthe amygdali
Expression system: Escherichia coli
UniProt:
  • Canonical: A2PZA5 (Residues: 1-344; Coverage: 48%)
Gene names: PaFS, orf1
Sequence domains: Terpene synthase family 2, C-terminal metal binding
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 143.295Å b: 143.295Å c: 118.261Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.178 0.206
Expression system: Escherichia coli