5epi

X-ray diffraction
4.1Å resolution

CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Polymerase acidic protein Chains: A, E, I, M, Q, U
Molecule details ›
Chains: A, E, I, M, Q, U
Length: 751 amino acids
Theoretical weight: 85.82 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chains: B, F, J, N, R, V
Molecule details ›
Chains: B, F, J, N, R, V
Length: 772 amino acids
Theoretical weight: 86.21 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chains: C, G, K, O, S, W
Molecule details ›
Chains: C, G, K, O, S, W
Length: 798 amino acids
Theoretical weight: 90.86 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8X3 (Residues: 1-770; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
CRNA 5' END Chains: D, H, L, P, T, X
Molecule details ›
Chains: D, H, L, P, T, X
Length: 12 nucleotides
Theoretical weight: 3.92 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 193.73Å b: 209.98Å c: 210.6Å
α: 117.71° β: 92.81° γ: 113.68°
R-values:
R R work R free
0.258 0.257 0.287
Expression system: Trichoplusia ni