› 5emb

X-ray diffraction
0.85Å resolution

Crystal structure of the SNX27 PDZ domain bound to the C-terminal phosphorylated PTHR PDZ binding motif

Released:
DOI: 10.2210/pdb5emb/pdb
PDB entry 5emb coloured by chain, front view.
PDB entry 5emb coloured by chain, side view.
PDB entry 5emb coloured by chain, top view.
Source organisms:
Primary publication:
A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex.
Clairfeuille T, Mas C, Chan AS, Yang Z, Tello-Lafoz M, Chandra M, Widagdo J, Kerr MC, Paul B, Mérida I, Teasdale RD, Pavlos NJ, Anggono V, Collins BM
Nat Struct Mol Biol 23 921-932 (2016)
PMID: 27595347

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169891 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sorting nexin-27 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 10.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K4V4 (Residues: 39-133; Coverage: 18%)
Gene names: Mrt1, Snx27
Sequence domains: PDZ domain
Parathyroid hormone/parathyroid hormone-related peptide receptor Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q03431 (Residues: 586-593; Coverage: 1%)
Gene names: PTH1R, PTHR, PTHR1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 37.57Å b: 48.33Å c: 56.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.124 0.136
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

To degrade or not to degrade: mechanisms and significance of endocytic recycling.
Cullen et al. (2018)
29 more

2 mentions without citation

Toward Understanding the Molecular Role of SNX27/Retromer in Human Health and Disease.
Chandra et al. (2021)
1 more