› 5eju

X-ray diffraction
1.65Å resolution

Ensemble refinement of the Crystal Structure of the Reversibly photoswitching chromoprotein Dathail, Ground State

Released:
DOI: 10.2210/pdb5eju/pdb
PDB 5eju coloured by chain and viewed from the front.
PDB 5eju coloured by chain and viewed from the side.
PDB 5eju coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
Evolution and characterization of a new reversibly photoswitching chromogenic protein, Dathail.
Langan PS, Close DW, Coates L, Rocha RC, Ghosh K, Kiss C, Waldo G, Freyer J, Kovalevsky A, Bradbury AR
J Mol Biol 428 1776-89 (2016)
PMID: 27000644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reversibly photoswitching protein Dathail Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.9 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 1 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 75.97Å b: 81.09Å c: 39.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.194
Expression system: Escherichia coli BL21

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Citations

2 review citations

Neutron Crystallography for the Study of Hydrogen Bonds in Macromolecules.
Oksanen et al. (2017)
1 more