5ejg

X-ray diffraction
2.88Å resolution

Crystal structure of NAD kinase P252D mutant from Listeria monocytogenes

Released:
Source organism: Listeria monocytogenes
Entry authors: Poncet-Montange G, Assairi L, Gelin M, Pochet S, Labesse G

Function and Biology Details

Reaction catalysed:
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 272 amino acids
Theoretical weight: 31.06 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y8D7 (Residues: 1-264; Coverage: 100%)
Gene names: lmo0968, nadK1
Sequence domains: ATP-NAD kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 67.217Å b: 119.655Å c: 68.624Å
α: 90° β: 100.92° γ: 90°
R-values:
R R work R free
0.22 0.217 0.324
Expression system: Escherichia coli BL21(DE3)