› 5eik

X-ray diffraction
2.3Å resolution

Structure of a Trimeric Intracellular Cation channel from C. elegans in the absence of Ca2+

Released:
DOI: 10.2210/pdb5eik/pdb
PDB entry 5eik coloured by chain, front view.
PDB entry 5eik coloured by chain, side view.
PDB entry 5eik coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Pore architecture of TRIC channels and insights into their gating mechanism.
Yang H, Hu M, Guo J, Ou X, Cai T, Liu Z
Nature 538 537-541 (2016)
PMID: 27698420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimeric intracellular cation channel type 1B.2 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.15 KDa
Source organism: Caenorhabditis elegans
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: Q9NA73 (Residues: 1-252; Coverage: 81%)
Gene names: Y57A10A.28, tric-1B.2
Sequence domains: TRIC channel

Ligands and Environments

5 bound ligands:
Ligand PT5 1 x PT5
Ligand CPL 1 x CPL
Ligand MG 1 x MG
Ligand ACT 5 x ACT
Ligand DMU 4 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 126.34Å b: 126.34Å c: 135.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.249
Expression system: Komagataella phaffii GS115

Quick links

Citations

7 review citations

Voltage-Sensing Phosphatases: Biophysics, Physiology, and Molecular Engineering.
Okamura et al. (2018)
6 more

3 mentions without citation

Crystal structures of the TRIC trimeric intracellular cation channel orthologues.
Kasuya et al. (2016)
2 more