PDBe 5eib

X-ray diffraction
2.1Å resolution

Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chain: C
Molecule details ›
Chain: C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chain: D
Molecule details ›
Chain: D
Length: 445 amino acids
Theoretical weight: 49.98 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Designed ankyrin repeat protein Chain: E
Molecule details ›
Chain: E
Length: 169 amino acids
Theoretical weight: 18 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Centromere protein J Chain: F
Molecule details ›
Chain: F
Length: 23 amino acids
Theoretical weight: 2.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9HC77 (Residues: 372-394; Coverage: 2%)
Gene names: CENPJ, CPAP, LAP, LIP1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 73.856Å b: 91.116Å c: 83.31Å
α: 90° β: 96.97° γ: 90°
R-values:
R R work R free
0.18 0.178 0.222
Expression systems:
  • Escherichia coli
  • Not provided