5ego

X-ray diffraction
2.54Å resolution

HOXB13-MEIS1 heterodimer bound to methylated DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein Meis1 Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00470 (Residues: 279-333; Coverage: 14%)
Gene name: MEIS1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
Homeobox protein Hox-B13 Chain: B
Molecule details ›
Chain: B
Length: 63 amino acids
Theoretical weight: 7.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92826 (Residues: 217-277; Coverage: 22%)
Gene name: HOXB13
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(P*GP*TP*TP*GP*AP*CP*AP*GP*TP*TP*TP*TP*AP*(5CM)P*GP*AP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.6 KDa
DNA (5'-D(*CP*CP*TP*(5CM)P*GP*TP*AP*AP*AP*AP*CP*TP*GP*TP*CP*AP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.46 KDa

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 40.976Å b: 51.48Å c: 113.575Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.265
Expression system: Escherichia coli BL21(DE3)