PDBe 5egj

X-ray diffraction
2.4Å resolution

The structural and biochemical characterization of acyl-coa hydrolase mutant Asn28Ala from Staphylococcus aureus in complex with COENZYME A

Released:
Entry authors: Khandokar YB, Srivastava PS, Forwood JK

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HotDog ACOT-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.22 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3K033 (Residues: 1-176; Coverage: 100%)
Gene name: SAV1878
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P6322
Unit cell:
a: 127.73Å b: 127.73Å c: 55.722Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.257
Expression system: Escherichia coli BL21(DE3)