5ed1

X-ray diffraction
2.77Å resolution

Human Adenosine Deaminase Acting on dsRNA (ADAR2) mutant E488Q bound to dsRNA sequence derived from S. cerevisiae BDF2 gene

Released:

Function and Biology Details

Reaction catalysed:
Adenine in double-stranded RNA + H(2)O = hypoxanthine in double-stranded RNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Double-stranded RNA-specific editase 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 403 amino acids
Theoretical weight: 45 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P78563 (Residues: 299-741; Coverage: 54%)
  • Best match: P78563-4 (Residues: 327-729)
Gene names: ADAR2, ADARB1, DRADA2, RED1
Sequence domains: Adenosine-deaminase (editase) domain
RNA (5'-R(*UP*UP*CP*CP*CP*CP*AP*CP*AP*UP*UP*(8AZ)P*GP*AP*CP*GP*UP*UP*CP*AP*GP*UP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
RNA (5'-R(*GP*AP*CP*UP*GP*AP*AP*CP*GP*AP*CP*CP*AP*AP*UP*GP*UP*GP*GP*GP*GP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.49 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 82.362Å b: 107.498Å c: 121.097Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.223
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided