› 5ebl

X-ray diffraction
2.3Å resolution

KcsA T75G in the Conductive State

Released:
DOI: 10.2210/pdb5ebl/pdb
PDB entry 5ebl coloured by chain, front view.
PDB entry 5ebl coloured by chain, side view.
PDB entry 5ebl coloured by chain, top view.
Source organisms:
Primary publication:
Individual Ion Binding Sites in the K(+) Channel Play Distinct Roles in C-type Inactivation and in Recovery from Inactivation.
Matulef K, Annen AW, Nix JC, Valiyaveetil FI
Structure 24 750-761 (2016)
PMID: 27150040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229864 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Fab Fragment Light Chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Antibody Fab Fragment Light Chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 125 amino acids
Theoretical weight: 13.32 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-125; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand F09 1 x F09
Ligand DGA 1 x DGA
Ligand K 6 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: I4
Unit cell:
a: 155.41Å b: 155.41Å c: 76.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.242
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

4 review citations

Chemoenzymatic Semisynthesis of Proteins.
Thompson et al. (2020)
3 more

2 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
1 more