› 5ebc

X-ray diffraction
3Å resolution

Crystal structure of EccB1 of Mycobacterium tuberculosis in spacegroup P21 (state III)

Released:
DOI: 10.2210/pdb5ebc/pdb
PDB entry 5ebc coloured by chain, front view.
PDB entry 5ebc coloured by chain, side view.
PDB entry 5ebc coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis.
Xie XQ, Zhang XL, Qi C, Li DF, Fleming J, Wang DC, Bi LJ
Acta Crystallogr F Struct Biol Commun 72 139-44 (2016)
PMID: 26841765

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESX-1 secretion system ATPase EccB1 Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 45.7 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNR7 (Residues: 72-480; Coverage: 85%)
Gene names: Rv3869, eccB1
Sequence domains: Type VII secretion system ESX-1, transport TM domain B
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 31.61Å b: 120.21Å c: 61.26Å
α: 90° β: 102.92° γ: 90°
R-values:
R R work R free
0.237 0.232 0.286
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Modeling Tubercular ESX-1 Secretion Using Mycobacterium marinum.
Chirakos et al. (2020)
1 other article

1 mention without citation

Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis.
Xie et al. (2016)