› 5e9f

X-ray diffraction
2.8Å resolution

Structural insights of isocitrate lyases from Magnaporthe oryzae

Released:
DOI: 10.2210/pdb5e9f/pdb
PDB entry 5e9f coloured by chain, front view.
PDB entry 5e9f coloured by chain, side view.
PDB entry 5e9f coloured by chain, top view.
Source organism: Pyricularia oryzae 70-15
Primary publication:
Crystal structure and functional analysis of isocitrate lyases from Magnaporthe oryzae and Fusarium graminearum.
Park Y, Cho Y, Lee YH, Lee YW, Rhee S
J Struct Biol 194 395-403 (2016)
PMID: 27016285

Function and Biology Details

Reactions catalysed: 
Isocitrate = succinate + glyoxylate
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 560 amino acids
Theoretical weight: 62.64 KDa
Source organism: Pyricularia oryzae 70-15
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CT06 (Residues: 1-547; Coverage: 100%)
Gene names: ICL1, MGG_04895
Sequence domains: Isocitrate lyase family

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 121.583Å b: 135.314Å c: 158.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.226
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Virulence function of the Ustilago maydis sterol carrier protein 2.
Krombach et al. (2018)
4 more