5e8j

X-ray diffraction
2.35Å resolution

Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with RAM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA cap guanine-N7 methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 36.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43148 (Residues: 165-476; Coverage: 66%)
Gene names: KIAA0398, RNMT
Structure domains: Vaccinia Virus protein VP39
RNA guanine-N7 methyltransferase activating subunit Chains: C, D
Molecule details ›
Chains: C, D
Length: 44 amino acids
Theoretical weight: 5.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BTL3 (Residues: 2-45; Coverage: 37%)
Gene names: C15orf18, FAM103A1, RAMAC, RAMMET

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 49.147Å b: 50.487Å c: 84.579Å
α: 90.06° β: 92.41° γ: 115.41°
R-values:
R R work R free
0.213 0.21 0.268
Expression system: Escherichia coli