› 5e5u

X-ray diffraction
2Å resolution

Crystal structure of the complex between Carbonic anhydrase-like domain of PTPRG and Immunoglobulin domains 2-3 of CNTN6

Released:
DOI: 10.2210/pdb5e5u/pdb
PDB entry 5e5u coloured by chain, front view.
PDB entry 5e5u coloured by chain, side view.
PDB entry 5e5u coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural Basis for Interactions Between Contactin Family Members and Protein-tyrosine Phosphatase Receptor Type G in Neural Tissues.
Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S
J Biol Chem 291 21335-21349 (2016)
PMID: 27539848

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170266 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor-type tyrosine-protein phosphatase gamma Chains: A, C
Molecule details ›
Chains: A, C
Length: 264 amino acids
Theoretical weight: 30.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05909 (Residues: 57-320; Coverage: 19%)
Gene name: Ptprg
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase
Contactin-6 Chains: B, D
Molecule details ›
Chains: B, D
Length: 199 amino acids
Theoretical weight: 22.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JMB8 (Residues: 119-316; Coverage: 20%)
Gene name: Cntn6
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

5 bound ligands:
Ligand 1PS 4 x 1PS
Ligand FMT 3 x FMT
Ligand MLI 1 x MLI
Ligand ACY 2 x ACY
Ligand MLT 4 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 78.641Å b: 113.53Å c: 117.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.217
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Conserved and Diverse Traits of Adhesion Devices from Siphoviridae Recognizing Proteinaceous or Saccharidic Receptors.
Goulet et al. (2020)
8 more

2 mentions without citation

Latrophilin's Social Protein Network.
Burbach et al. (2019)
1 more