5e5p

X-ray diffraction
2.65Å resolution

Wild type I-SmaMI in the space group of C121

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
I-SmaMI LAGLIDADG meganuclease Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.15 KDa
Source organism: Sordaria macrospora k-hell
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7WD42 (Residues: 114-415; Coverage: 70%)
Gene name: SMAC_12671
Sequence domains: LAGLIDADG endonuclease

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 148.327Å b: 58.23Å c: 42.046Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.179 0.175 0.265
Expression system: Escherichia coli BL21(DE3)