PDBe 5e5o

X-ray diffraction
2.36Å resolution

I-SmaMI bound to uncleaved DNA target in the presence of Calcium ions

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
I-SmaMI LAGLIDADG endonuclease Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.16 KDa
Source organism: Sordaria macrospora k-hell
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7WD42 (Residues: 114-415; Coverage: 70%)
Gene name: SMAC_12671
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (25-MER) Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 7.69 KDa
Source organism: synthetic construct
Expression system: Not provided
Top strand DNA (25-MER) Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 61.327Å b: 67.993Å c: 97.743Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.274
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided