5e08

X-ray diffraction
2.38Å resolution

Specific Recognition of a Single-stranded RNA Sequence by an Engineered Synthetic Antibody Fragment

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 24.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
RNA Chain: N
Molecule details ›
Chain: N
Length: 12 nucleotides
Theoretical weight: 3.85 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 199.515Å b: 63.401Å c: 57.061Å
α: 90° β: 97.51° γ: 90°
R-values:
R R work R free
0.211 0.209 0.234
Expression system: Escherichia coli