› 5dv9

X-ray diffraction
2.4Å resolution

Crystal structure of the Luciferase

Released:
DOI: 10.2210/pdb5dv9/pdb
PDB entry 5dv9 coloured by chain, front view.
PDB entry 5dv9 coloured by chain, side view.
PDB entry 5dv9 coloured by chain, top view.
Source organism: Photinus pyralis
Entry authors: Su J, Li Z, Yuan Z, Gu L

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 573 amino acids
Theoretical weight: 63.37 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 117.967Å b: 117.967Å c: 95.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.241
Expression system: Escherichia coli

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