› 5dov

X-ray diffraction
1.8Å resolution

Crystal structure of JamJ enoyl reductase (apo form)

Released:
DOI: 10.2210/pdb5dov/pdb
PDB entry 5dov coloured by chain, front view.
PDB entry 5dov coloured by chain, side view.
PDB entry 5dov coloured by chain, top view.
Source organism: Lyngbya majuscula
Primary publication:
Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.
Khare D, Hale WA, Tripathi A, Gu L, Sherman DH, Gerwick WH, Håkansson K, Smith JL
Structure 23 2213-2223 (2015)
PMID: 26526850

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
JamJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 40.39 KDa
Source organism: Lyngbya majuscula
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6E7K0 (Residues: 260-594; Coverage: 10%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 50.905Å b: 57.166Å c: 66.132Å
α: 95.27° β: 106.75° γ: 105.12°
R-values:
R R work R free
0.186 0.184 0.223
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The architectures of iterative type I PKS and FAS.
Herbst et al. (2018)
3 more

2 mentions without citation

Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.
Khare et al. (2015)
1 more