PDBe 5do9

X-ray diffraction
2.6Å resolution

Structure of regulator of G protein signaling 8 (RGS8) in complex with AlF4-activated Galpha-q


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(q) subunit alpha Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 314 amino acids
Theoretical weight: 36.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P21279 (Residues: 37-350; Coverage: 88%)
Gene name: Gnaq
Structure domains:
Regulator of G-protein signaling 8 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 134 amino acids
Theoretical weight: 15.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P57771 (Residues: 42-173; Coverage: 73%)
Gene name: RGS8
Sequence domains: Regulator of G protein signaling domain
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 173.946Å b: 95.881Å c: 112.898Å
α: 90° β: 94.31° γ: 90°
R R work R free
0.18 0.178 0.226
Expression system: Escherichia coli BL21(DE3)