› 5dns

X-ray diffraction
3.56Å resolution

t1428 loop variant in P3221

Released:
DOI: 10.2210/pdb5dns/pdb
PDB entry 5dns coloured by chain, front view.
PDB entry 5dns coloured by chain, side view.
PDB entry 5dns coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.
MacDonald JT, Kabasakal BV, Godding D, Kraatz S, Henderson L, Barber J, Freemont PS, Murray JW
Proc Natl Acad Sci U S A 113 10346-51 (2016)
PMID: 27573845

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
t1428 beta1 loop design Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 25.69 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: E3 ubiquitin-protein ligase SopA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 104.57Å b: 104.57Å c: 87.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.184 0.284
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Computational protein design with backbone plasticity.
MacDonald et al. (2016)
6 more

2 mentions without citation

Designed for life: biocompatible de novo designed proteins and components.
Grayson et al. (2018)
1 more