› 5dkk

X-ray diffraction
2.5Å resolution

Structure of the dark-state monomer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum

Released:
DOI: 10.2210/pdb5dkk/pdb
PDB entry 5dkk coloured by chain, front view.
PDB entry 5dkk coloured by chain, side view.
PDB entry 5dkk coloured by chain, top view.
Source organism: Phaeodactylum tricornutum
Primary publication:
Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence.
Heintz U, Schlichting I
Elife 5 e11860 (2016)
PMID: 26754770

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LOV domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.01 KDa
Source organism: Phaeodactylum tricornutum
Expression system: Escherichia coli
UniProt:
  • Canonical: B7G9J2 (Residues: 1-103; Coverage: 100%)
Gene name: PHATRDRAFT_8113
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

3 bound ligands:
Ligand FMN 2 x FMN
Ligand ACT 2 x ACT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P212121
Unit cell:
a: 64.37Å b: 69.21Å c: 74.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.254
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Blue-Light Receptors for Optogenetics.
Losi et al. (2018)
13 more

9 mentions without citation

Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence.
Heintz et al. (2016)
8 more