› 5dh7

X-ray diffraction
3.06Å resolution

Two divalent metal ions and conformational changes play roles in the hammerhead ribozyme cleavage reaction-G12A mutant in Mn2+

Released:
DOI: 10.2210/pdb5dh7/pdb
PDB 5dh7 coloured by chain and viewed from the front.
PDB 5dh7 coloured by chain and viewed from the side.
PDB 5dh7 coloured by chain and viewed from the top.
Source organism: synthetic construct
Entry authors: Mir A, Chen J, Neau D, Golden BL

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (48-MER) Chain: A
Molecule details ›
Chain: A
Length: 48 nucleotides
Theoretical weight: 15.48 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-R(*GP*GP*GP*CP*GP*UP*DC*UP*GP*GP*GP*CP*AP*GP*UP*AP*CP*CP*CP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.44 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 11 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 81.309Å b: 85.825Å c: 103.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.252
Expression system: Not provided

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Citations

5 review citations

Self-cleaving ribozymes: substrate specificity and synthetic biology applications.
Peng et al. (2021)
4 more