5dh7

X-ray diffraction
3.06Å resolution

Two divalent metal ions and conformational changes play roles in the hammerhead ribozyme cleavage reaction-G12A mutant in Mn2+

Released:
Source organism: synthetic construct
Entry authors: Mir A, Chen J, Neau D, Golden BL

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (48-MER) Chain: A
Molecule details ›
Chain: A
Length: 48 nucleotides
Theoretical weight: 15.48 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-R(*GP*GP*GP*CP*GP*UP*DC*UP*GP*GP*GP*CP*AP*GP*UP*AP*CP*CP*CP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.44 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 81.309Å b: 85.825Å c: 103.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.252
Expression system: Not provided