PDBe 5d9d

X-ray diffraction
1.7Å resolution

Luciferin-regenerating enzyme solved by SAD using synchrotron radiation at room temperature

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SGL domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.2 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95YI4 (Residues: 1-308; Coverage: 100%)
Sequence domains: SMP-30/Gluconolactonase/LRE-like region
Structure domains: TolB, C-terminal domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 48.107Å b: 77.177Å c: 84.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.178
Expression system: Escherichia coli BL21(DE3)