5d8l

X-ray diffraction
2.07Å resolution

Human HSF2 DNA Binding Domain in complex with 3-site HSE DNA at 2.1 Angstroms Resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heat shock factor protein 2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 110 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03933 (Residues: 8-115; Coverage: 20%)
Gene names: HSF2, HSTF2
Sequence domains: HSF-type DNA-binding
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(P*GP*TP*GP*AP*AP*TP*AP*TP*TP*CP*TP*AP*GP*AP*AP*CP*C)-3') Chains: A, G
Molecule details ›
Chains: A, G
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*TP*CP*TP*AP*GP*AP*AP*TP*AP*TP*TP*CP*AP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 40.174Å b: 47.14Å c: 109.587Å
α: 82.82° β: 90.03° γ: 64.75°
R-values:
R R work R free
0.22 0.217 0.273
Expression systems:
  • Escherichia coli
  • Not provided